Metabolites change of Scenedesmus obliquus exerted by AgNPs

论文摘要

With increasing emission of silver nanoparticles（AgNPs） into the environment, it is important to understand the effects of ambient concentration of AgNPs. The biological effects of AgNPs on Scenedesmus obliquus, a ubiquitous freshwater microalgae, was evaluated. AgNPs exerted a minor inhibitory effect at low doses. Non-targeted metabolomic studies were conducted to understand and analyze the effect of AgNPs on algal cells from a molecular perspective. During the 48 hr of exposure to AgNPs, 30 metabolites were identified, of which nine had significant changes compared to the control group. These include D-galactose, sucrose, and D-fructose.These carbohydrates are involved in the synthesis and repair of cell walls. Glycine, an important constituent amino acid of glutathione, increased with AgNP exposure concentration increasing, likely to counteract an increased intracellular oxidative stress. These results provide a new understanding of the toxicity effects and mechanism of AgNPs. These metabolites could be useful biomarkers for future research, employed in the early detection of environmental risk from AgNPs.

论文目录

• Introduction
• 1. Materials and methods
•   1.1. Materials
•   1.2. Preparation and characterization of AgNPs
•   1.3. S.obliquus culture
•   1.4. Exposure of S.obliquus to AgNPs
•   1.5. Metabolomic profiling
•     1.5.1. Extraction and analysis of metabolites
•     1.5.2. Statistical analysis
• 2. Results and discussion
•   2.1. Growth inhibition of AgNPs on S.obliquus
•   2.2. Metabolite identification and function analysis
•   2.3. Speculated mechanism
• 3. Conclusions

文章来源

类型: 期刊论文

作者: Pu Wang,Bo Zhang,Hui Zhang,Yiliang He,Choon Nam Ong,Jun Yang

来源: Journal of Environmental Sciences 2019年02期

年度: 2019

分类: 工程科技Ⅰ辑

专业: 环境科学与资源利用

单位: School of Environmental Science & Engineering,Shanghai Jiaotong University,School of Municipal and Environmental Engineering,Lanzhou Jiaotong University,NUS Environmental Research Institute,National University of Singapore

基金: supported by the National Natural Science Foundation of China (No.21677097),the National Science and Technology Major Projects of Water Pollution Control and Management of China (No.2014ZX07206001),the National Research Foundation (NRF),Singapore under its Campus for Research Excellence and Technological Enterprise (CREATE) Program

分类号: X174;X52

页码: 310-318

总页数: 9

文件大小: 415K

下载量: 14