Persistence of Cry1Ac Protein from Transgenic Bt Cotton Cultivation and Residue Returning in Fields and Its Effect on Functional Diversity of Soil Microbial Communities

论文摘要

The persistence of Cry1 Ac protein in the soil and its effect on soil microbial communities are a core issue in assessing the ecological risk of transgenic Bacillus thuringiensis（Bt） cotton. In this study a field experiment was conducted on the cultivation of transgenic Bt cotton（Jin 26 and BtJ i 668） with the immediate returning of residues to the fields, in order to quantify the Cry1 Ac protein content in the fields and investigate its effects on the functional diversity of soil microbial communities. Cry1 Ac protein in the residue-soil mixture was gradually degraded in the transgenic Bt cotton fields. After transgenic Bt cotton straw was returned to the fields for 30 d, 63.73%and 58.33% of the initial amounts of Cry1 Ac protein were degraded in the Jin 26 and BtJ i 668 fields, respectively. Before the crops were sown in the following year（180 d after returning the straw）, no Cry1 Ac protein was detected in the fields. After returning the cotton straw to the fields for 30 d, the Shannon-Wiener and McIntosh indices of soil microbial communities in the transgenic Bt cotton fields were significantly higher than those in the non-transgenic cotton fields. Meanwhile, the utilization of carbon sources including amino acids, amines, and carbohydrates by the soil microbial communities significantly increased. Both the McIntosh index and the utilization of carbohydrates increased until 180 d. Principal component analysis revealed that amino acids, amides, and carbohydrates were the main carbon sources distinguishing the two principal component factors. These findings indicated that Cry1 Ac protein did not accumulate in the fields after transgenic Bt cotton was planted for one year and the residues were immediately returned to the fields; however, the original functional diversity of soil microbial communities was affected continuously.

论文目录

• INTRODUCTION
• MATERIALS AND METHODS
•   Experimental site and crop
•   Plot experiment
•   Soil sampling
•   Cry1Ac protein determination
•   Biolog test
•   Data analysis
• RESULTS AND DISCUSSION
•   Diversity indices of soil microbial community
•   Utilization of carbon sources by soil microbial communities
•   Principal component analysis
• CONCLUSIONS

文章来源

类型: 期刊论文

作者: ZHANG Meijun,FENG Meichen,XIAO Lujie,SONG Xiaoyan,Guangwei DING,YANG Wude

来源: Pedosphere 2019年01期

年度: 2019

分类: 农业科技,基础科学

专业: 生物学,农业基础科学,农艺学

单位: College of Agriculture, Shanxi Agricultural University,Department of Chemistry, Northern State University

基金: supported by the Shanxi Provincial Natural Science Foundation of China (No. 2012011030-2),181 Cultivate Foundation of National Natural Science from Shanxi Agricultural University (No. 2017GPY07)

分类号: S154.3

页码: 114-122

总页数: 9

文件大小: 215K

下载量: 41