一种用于增强光动力/光热协同肿瘤治疗的酸性刺激响应锌(Ⅱ)金属卟啉化合物（英文）

论文摘要

肿瘤酸性微环境是低氧条件下糖酵解引发的,它能激发体系的酸性响应系统,为肿瘤靶向光治疗建立一定的触发机制.金属化卟啉结构具有单态氧产率高、荧光成像能力强等优点,在生物医学领域得到广泛研究.本文设计合成了一种具有优异荧光量子产率（67.4%）、高单态氧量子产率（84.3%）和理想光热转换效率（30.0%）的酸性响应锌（II）金属卟啉（P-4）,研究了其酸性肿瘤微环境刺激响应光动力/光热治疗性能.自组装的P-4纳米粒子可以特异性地靶向溶酶体亚细胞器位点,实现二丁胺苯基（DBAP）的质子化过程且伴随高光毒性;在660 nm单激光照射下,肿瘤被完全消除且无副作用,表明酸刺激响应的P-4纳米粒子可以为肿瘤协同治疗提供一种新途径.

论文目录

• INTRODUCTION
• EXPERIMENTAL SECTION
•   Materials
•   Characterizations
•   Synthesis of P-4
•   Preparation and characterizations of P-4 NPs
•   Cell culture and incubation condition
•   In vitro cell cytotoxicity
•   Apoptosis study by flow cytometry
•   AM/PI staining
•   In vitro cellular uptake of P-4 NPs
•   Intracellular ROS assay and sub-cellular localization
•   In vivo fluorescence imaging
•   Pharmacokinetics
•   In vivo antitumor assay
•   Histology sample preparation
• RESULTS
•   Synthesis and characterization
•   pH-responsive performance
•   In vitro examinations
•   In vivo examinations
• CONCLUSIONS

文章来源

类型: 期刊论文

作者: 梁平平,汤昊,顾瑞,薛磊,陈大鹏,王文军,杨洲,司伟丽,董晓臣

来源: Science China Materials 2019年08期

年度: 2019

分类: 工程科技Ⅰ辑,医药卫生科技

专业: 化学,肿瘤学

单位: Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech),Department of Pharmaceutical Preparation, Jinling Hospital,School of Physical Science and Information Technology, Liaocheng University,Beijing Key Laboratory of Function Materials for Molecule & Structure Construction, School of Materials Science and Engineering, University of Science and Technology Beijing

基金: supported by the National Natural Science Foundation of China(61525402,61775095 and 21704043),Jiangsu Provincial Key Research and Development Plan(BE2017741),Six Talent Peak Innovation Team in Jiangsu Province(TD-SWYY-009),the Natural Science Foundation of Jiangsu Province(BK20170990and 17KJB150020)

分类号: O641.4;R73-36

页码: 1199-1209

总页数: 11

文件大小: 1411K

下载量: 301