论文摘要
Class Ⅱ major histocompatibility complex（MHC-Ⅱ） transactivator（CIITA） is a member of the pattern recognition receptor in cytoplasm,which is involved in host innate immune responses.In this study,the full-length cDNA of Cherry Valley duck CIITA（duCIITA） was cloned from the spleen of healthy Cherry Valley ducks for the first time.The CDs of duCIITA have 3648 bp and encode 1215 amino acids.The homology analysis of CIITAs amino acid sequence showed that the duCIITA has the highest identity with the Anas platyrhynchos（94.9%）,followed by Gallus gallus and Meleagris gallopavo.Quantitative real-time PCR analysis indicated that duCIITA mRNA has a broad expression level in healthy Cherry Valley duck tissues.It was highly expressed in the lung and cerebellum,and lowly expressed in the rectum and esophagus.After the avian pathogenic Escherichia coli（APEC） O1 K1 infection,the ducks exhibited the typical clinical symptoms,and a severe fibrinous exudate in the heart and liver surface was observed.Meanwhile,a significant up-regulation of duCIITA was detected in the infected liver.The inflammatory cytokines IL-1β,IL-6 and IL-8 have a significant up-regulation in the infected liver,spleen and brain.In addition,knockdown of the duCIITA reduces antibacterial activity and inflammatory cytokine production of the duck embryo fibroblast cells.Our research is the first study of the cloning,tissue distribution,and antibacterial immune responses of duCIITA,and these findings imply that duCIITA was an important receptor,which was involved in the early stage of the antibacterial innate immune response to APEC O1 K1 infection of Cherry Valley duck.
论文目录
文章来源
类型: 国际会议
作者: Rong Li,Tongjie Chai,Liangmeng Wei
来源: 世界动物福利科学大会 2019-05-28
年度: 2019
分类: 基础科学,农业科技
专业: 生物学,畜牧与动物医学
单位: College of Veterinary Medicine,Shandong Agricultural University,Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong ProvinceCollaborative innovation center for the origin and control of emerging infectious diseases,Taishan Medical UniversityShandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention,Shandong Agricultural UniversityShandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention,Shandong Agricultural University
分类号: S852.61
DOI: 10.26914/c.cnkihy.2019.033759
页码: 196-207
总页数: 12
文件大小: 2598k
下载量: 1