导读:本文包含了肝血管生成论文开题报告文献综述及选题提纲参考文献，主要关键词:胎盘生长因子,慢性肝病,肝纤维化,肝硬化
肝血管生成论文文献综述
李希,涂传涛[1]（2016）在《胎盘生长因子在慢性肝病肝血管生成中的研究进展》一文中研究指出慢性肝病相关的肝硬化曲张静脉出血和原发性肝癌的预后极差,临床上缺乏治疗手段。近年来发现血管生成在慢性肝病形成与进展中发挥重要的作用,胎盘生长因子仅参与病理性血管生成,作为抗血管生成治疗的潜在靶点具有显着优势。本文就胎盘生长因子在慢性肝病肝血管生成中的研究现状进行综述。(本文来源于《中国肝脏病杂志(电子版)》期刊2016年03期）
Khaled,Abdel-Hakim,Nagwa,El-Nefiawy[2]（2011）在《肝血管生成:在非转移性结肠直肠癌中肿瘤宿主的相互作用(英文)》一文中研究指出Objective: Angiogenesis is a crucial step for tumor growth and progression. Changes of liver angiogenesis (without metastatic invasion) in response to primary tumors are not known. The aim of the study was to investigate the liver angiogenesis in non-metastatic colorectal cancer (CRC). Methods: Human colorectal adenocarcinoma tumors were grown subcutaneously in nude mice. All animals showed tumor growth locally without macroscopic or microscopic evidence of liver metastases. Livers were investigated for their microvessel density (MVD) at different stages of tumor growth (as small, medium, and large-sized tumors). Normal non-tumor-bearing mice served as controls. To assess MVD, two endothelial cell markers (anti-CD34 and -CD31 antibodies), image analysis, and immunofluorescent technique were utilized. Enumeration of positive stained endothelial cells revealed the MVD. Results: Non-metastatic livers showed increased levels of MVD vs. control. Moreover, levels of MVD were higher in small and medium-sized tumor groups versus large sized tumor groups. Conclusion: The present data indicate that angiogenesis in the liver is induced in early-stages of CRC. However, this effect is suppressed with advanced tumor growth. These results provide an additional rationale for including antiangiogenic therapy in the treatment of early stages of CRC.(本文来源于《Chinese-German Journal of Clinical Oncology》期刊2011年10期）
严家春,陈文笔,马勇,徐长江[3]（2000）在《四氯化碳诱导鼠肝血管生成因子免疫组织化学观察》一文中研究指出目的研究 CCl_4诱导鼠肝血管生成因子表达与肝纤维化(LF)及肝硬变(LC)病变的关系.方法 SD 雄性6mo 龄鼠50只,随机分成2组,40只用于造模,10只为对照.实验鼠以3mL·kg~(-1)皮下多点注射500mL·L~(-1)CCl_4(用液状石蜡配制),2次·wk~(-1)对照组不处理.分别于实验2,5,8及12wk 解剖造模鼠10只,作冰冻、石蜡切片及VEGF,α-SMA 及ⅧR Ag 免疫组化观察.结果实验2 wk 鼠体质量略下降而肝质量增加,5 wk 始肝质量下降,至8 wk～12 wk 最明显(P<0.01),且肝表面伴结节形成,组织学证实已发生 LC.免疫组化显示,在实验2 wk VEGF 阳性肝细胞局限于病变周围,部分肝窦内皮细胞α-SMA,ⅧR Ag示阳性表达,以后随肝血管病变加重而上述因子表达强度及分布范围渐增加,提示两者关系密切.结论肝细胞在 LF 及 LC 发展中担当一重要角色.(本文来源于《世界华人消化杂志》期刊2000年11期）
肝血管生成论文开题报告
（1）论文研究背景及目的
此处内容要求：
首先简单简介论文所研究问题的基本概念和背景，再而简单明了地指出论文所要研究解决的具体问题，并提出你的论文准备的观点或解决方法。
写法范例：
Objective: Angiogenesis is a crucial step for tumor growth and progression. Changes of liver angiogenesis (without metastatic invasion) in response to primary tumors are not known. The aim of the study was to investigate the liver angiogenesis in non-metastatic colorectal cancer (CRC). Methods: Human colorectal adenocarcinoma tumors were grown subcutaneously in nude mice. All animals showed tumor growth locally without macroscopic or microscopic evidence of liver metastases. Livers were investigated for their microvessel density (MVD) at different stages of tumor growth (as small, medium, and large-sized tumors). Normal non-tumor-bearing mice served as controls. To assess MVD, two endothelial cell markers (anti-CD34 and -CD31 antibodies), image analysis, and immunofluorescent technique were utilized. Enumeration of positive stained endothelial cells revealed the MVD. Results: Non-metastatic livers showed increased levels of MVD vs. control. Moreover, levels of MVD were higher in small and medium-sized tumor groups versus large sized tumor groups. Conclusion: The present data indicate that angiogenesis in the liver is induced in early-stages of CRC. However, this effect is suppressed with advanced tumor growth. These results provide an additional rationale for including antiangiogenic therapy in the treatment of early stages of CRC.
（2）本文研究方法
调查法：该方法是有目的、有系统的搜集有关研究对象的具体信息。
观察法：用自己的感官和辅助工具直接观察研究对象从而得到有关信息。
实验法：通过主支变革、控制研究对象来发现与确认事物间的因果关系。
文献研究法：通过调查文献来获得资料，从而全面的、正确的了解掌握研究方法。
实证研究法：依据现有的科学理论和实践的需要提出设计。
定性分析法：对研究对象进行“质”的方面的研究，这个方法需要计算的数据较少。
定量分析法：通过具体的数字，使人们对研究对象的认识进一步精确化。
跨学科研究法：运用多学科的理论、方法和成果从整体上对某一课题进行研究。
功能分析法：这是社会科学用来分析社会现象的一种方法，从某一功能出发研究多个方面的影响。
模拟法：通过创设一个与原型相似的模型来间接研究原型某种特性的一种形容方法。
肝血管生成论文参考文献
[1].李希,涂传涛.胎盘生长因子在慢性肝病肝血管生成中的研究进展[J].中国肝脏病杂志(电子版).2016
[2].Khaled,Abdel-Hakim,Nagwa,El-Nefiawy.肝血管生成:在非转移性结肠直肠癌中肿瘤宿主的相互作用(英文)[J].Chinese-GermanJournalofClinicalOncology.2011
[3].严家春,陈文笔,马勇,徐长江.四氯化碳诱导鼠肝血管生成因子免疫组织化学观察[J].世界华人消化杂志.2000