论文摘要
野油菜黄单胞菌野菜致病变种（Xanthomonas campestris pv.campestris,Xcc）是严重威胁十字花科植物的重要病原真菌之一,可造成全世界范围内农业的严重减产。DNA拓扑异构酶是通过调节DNA的拓扑结构进而影响DNA复制、重组和转录过程。目前尚未见文献报道有关病原细菌中拓扑异构酶功能研究。为分析拓扑异构酶是否影响野油菜黄单胞菌野菜致病变种的致病性,本实验以Xcc 8004中Xcc0034基因,预测编码的TopoIB蛋白作为研究对象,通过对TopoIB蛋白的理化性质进行初步的生物信息学分析,表明TopoIB蛋白含有核定位信号和信号肽。在农杆菌介导烟草叶片中瞬时表达,发现p1305-GFPTopoIB融合蛋白定位于烟草叶片的细胞核上。构建topoIB基因的原核表达载体,纯化的TopoIB重组蛋白反应15 min可以完全解旋超螺旋DNA,表明TopoIB蛋白具有拓扑异构酶活性。将topoIB基因突变后得到突变体ATopoIB,表型检测结果显示,ΔTopoIB菌株与野生型Xcc 8004在植物体内和培养基中的生长状况无明显差异,且胞外多糖产量基本相同,此外胞外蛋白酶、胞外纤维素酶和胞外淀粉酶产量也与野生型Xcc 8004基本一致。在拟南芥和甘蓝（’京华一号’）上致病性检测显示ΔTopoIB菌株致病性并没有受到影响。因此表明topoIB与Xcc 8004的致病性无明显的直接关系。
论文目录
文章来源
类型: 国内会议
作者: 李珅瑀,张迎,王诚,孔维文,张清霞
关键词: 缺失突变,亚细胞定位,原核表达
来源: 中国植物病理学会2019年学术年会 2019-07-20
年度: 2019
分类: 基础科学,农业科技
专业: 生物学,农业基础科学,植物保护
单位: 扬州大学园艺与植物保护学院
分类号: S432.44
DOI: 10.26914/c.cnkihy.2019.020393
页码: 378
总页数: 1
文件大小: 43k
下载量: 25
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