A plant viral protein disrupts mitosis and contributes to host dwarfism by directly interrupting the conserved mitotic entry switch Wee1-Cdc25-Cdk1

论文摘要

Disruption of host mitosis plays an important role in viral pathogenesis However,the underlying molecular mechanisms are still not well elucidated.Here,we report that the 17 K protein,conserved among the grass-infecting barley yellow dwarf viruses（BYDVs） and related poleroviruses,disrupts G2/M transition and inhibits cell growth in both host（barley） and nonhost（fission yeast and Arabidopsis thaliand）organisms through directly interrupting the mitotic entry switch Weel-Cdc25-Cdkl（CDKA in plant and Cdc2 in fission yeast）.17 K binds to Cdc25 and CDKA/Cdc2 but not Weel,and decreases Cdc25’s phosphatase activity in vitro.Furthermore,17 K expression up-regulates Weel accumulation.These alterations lead to abnormal accumulation of the CDKA/Cdc2 phosphorylated at critical mitosis-regulating residues,which impedes the function of CDKA/Cdc2 and results in G2/M transition.Finally,perturbing 17 K-CDKA interaction by silencing CDKA expression aggravates BYDV-induced dwarfing symptom.Our work suggests that the 17 K protein is an important contributor to BYDV-induced host dwarfism.Manipulating the interaction between 17 K and Weel-Cdc25-CDKA may aid the control of cereal yellow dwarf diseases.Interestingly,direct interruption of Weel-Cdc25-Cdkl is also being found for an increasing number of animal viral proteins,indicating commonality in the molecular mechanism underlying the disruption of mitosis by plant and animal viruses.

论文目录

文章来源

类型: 国内会议

作者: Huaibing Jin,Zhiqiang Du,Yanjing Zhang,Judit Antal,Zongliang Xia,Yan Wang,Yang Gao,Xiaoge Zhao,Xinyun Han,Yanjun Cheng,Qianhua Shen,Kunpu Zhang,Robert E.Elder,Zsigmond Benko,Csaba Fenyvuesvolgyi,Ge Li,Dionne Rebello,Richard Y.Zhao,Daowen Wang

来源: 第十届全国小麦基因组学及分子育种大会 2019-08-11

年度: 2019

分类: 基础科学

专业: 生物学,生物学

单位: State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Chinese Academy of SciencesUniversity of Chinese Academy of SciencesCollege of Agronomy and State Key Laboratory of Wheat and Maize Crop Science,Henan Agricultural UniversityChicago Memorial Institute for Education and Research,Northwestern University Feinberg School of MedicineDepartment of Pathology,University of Maryland School of MedicineDepartment of Microbiology-Immunology,Institute of Human Virology,and Institute of Global Health,University of Maryland School of Medicine

分类号: Q943

DOI: 10.26914/c.cnkihy.2019.021191

页码: 312-313

总页数: 2

文件大小: 69k

下载量: 4

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