Simultaneous Determination of Six Constituents in Cangbaiqutong Capsule by HPLC Wavelength Switching Technique

论文摘要

[Objectives] To establish a high performance liquid chromatography（HPLC） wavelength switching method for the simultaneous determination of content of six constituents（phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol） in Cangbaiqutong capsules, and provide a scientific basis for the comprehensive evaluation of the quality of Cangbaiqutong capsule. [Methods] The chromatographic column of Waters XSELECT CSH-C18（4.6 mm × 150 mm, 5 μm）, the mobile phase of acetonitrile-0.1% phosphate solution, gradient elution（0-15 min,10%-18% A;15-30 min,18%-50% A; 30-35 min, 50%-10% A）; the flow rate of 1.0 mL/min, wavelength switching of 284（0-7 min, phellodendrine）, 274（7-10 min, gentiopicrin）, 230（10-14 min, paeoniflorin） and 274 nm（14-35 min, tetrandrine, berberine hydrochloride, paeonol）, the injection volume of 10 μL. [Results] There was a good linear relationship between the area of chromatographic peak and the injection volume of phellodendrine chloride, gentiopicrin, paeoniflorin, tetrandrine, berberine hydrochloride and paeonol in the range of 0.150-1.504, 0.768-7.680, 1.096-10.960, 0.220-2.200, 0.296-2.956, 0.0345-0.345 μg, respectively; the average recovery rates（n=6） were 98.3%, 99.2%, 98.8%, 98.8%, 99.1% and 98.2%, respectively; the RSD value was 1.32%, 1.46%, 1.08%, 1.31%, 1.26% and 1.21%, respectively. [Conclusions] The method can be used to determine many kinds of constituents at the same time, and the operation is simple, accurate and reproducible, and can be used for the quality control of compound Cangbaiqutong capsules.

论文目录

• 1 Introduction
• 2 Experiment
•   2.1 Instruments and reagents
•   2.2 Methods and results[1-6]
•     2.2.1 Chromatographic conditions.
•     2.2.2 Establishment of multi-wavelength switching.
•     2.2.3 Preparation of solution.
•     2.2.4 Specificity investigation.
•     2.2.5 Investigation of linear relationship.
•     2.2.6 Precision test.
•     2.2.7 Stability test.
•     2.2.8 Replicability test.
•     2.2.9 Recovery rate of samples.
•     2.2.10 Determination of sample content.
• 3 Discussions

文章来源

类型: 期刊论文

作者: Jing WU,Hongyan LIU,Bo YU,Zhong JIA

来源: Medicinal Plant 2019年04期

年度: 2019

分类: 农业科技,工程科技Ⅰ辑,医药卫生科技

专业: 化学,中药学

单位: Chinese Medicine Hospital of Lanzhou City,Lanzhou Pulmonary Hospital,The Second People’s Hospital of Lanzhou

基金: Supported by Lanzhou Talent Innovation and Entrepreneurship Science and Technology Plan Project(2015-RC-22)

分类号: R286.0;O657.72

DOI: 10.19600/j.cnki.issn2152-3924.2019.04.005

页码: 20-23

总页数: 4

文件大小: 343K

下载量: 12