Identification of Polyphenols from Unripe Apple with Viscozyme L Extraction

论文摘要

As a potential source of fine chemicals, unripe apple polyphenols were extracted with Viscozyme L treatment. And then, a preparative moderate pressure liquid chromatography（MPLC） method for separation and purification of polyphenols were successfully established by using silica gel column and octadesyl silane column. The structures of these compounds were identified by 1H and 13C NMR spectroscopy. The results indicated that Viscozyme L had the strongest effect on polyphenols extraction, the yield of apple crude polyphenols（ACP） was 7.08%, and the total phenolic content（TPC） of ACP was 0.95 g GAE/100 g, which were 2 times of that of control; 5 sub-fractions were obtained from ACP, which were A-1, A-2, and B-1, B-2, B-3; fractions A-2 and B-2 were identified as p-coumaric acid and caffeic acid and the yields were 111.54 and 161.70 mg/100 g ACP, respectively. In addition, p-coumaric acid and caffeic acid were the major components in Viscozyme L-aided polyphenols extraction, which could be explained by the result of degradation of lignin and chlorogenic acid by carbohydrate-hydrolyzing enzyme, Viscozyme L.

论文目录

• Materials and Methods
•   Materials
•   Reagents and instruments
•   Enrichment of polyphenols
•   Determination of total phenolic content
•   Isolation and purification unripe apple polyphenols
•   Identification of unripe apple polyphenols
•   Statistical analysis
• Results and Analysis
•   Extraction efficiency of unripe apple polyphenols with Viscozyme L treatment
•   Selective isolation of polyphenols
•   Identification of polyphenols
• Conclusions and Discussion

文章来源

类型: 期刊论文

作者: Huzhe ZHENG,Chunlan CUI,Shinkyo CHUNG

来源: Agricultural Biotechnology 2019年02期

年度: 2019

分类: 农业科技,工程科技Ⅰ辑

专业: 轻工业手工业

单位: Department of Food Science, Jiangsu Food & Pharmaceutical Science College,Department of Food Science and Technology, Kyungpook National University

基金: Supported by 333 Project in Jiangsu Province,Qing Lan Project in Jiangsu Province

分类号: TS255.1

DOI: 10.19759/j.cnki.2164-4993.2019.02.036

页码: 153-156

总页数: 4

文件大小: 688K

下载量: 25