Proteomic analysis reveals the differential proteins of endosperm between soft and hard wheat varieties

论文摘要

In this study, we revealed the differential proteins from the wheat endosperms using proteomic analysis and investigated their surface properties. The pattern of the polypeptides obtained from the Yangmai-15 and Yangmai-16 wheat varieties were compared using two-dimensional polyacrylamide gels. In addition, we compared the characteristics of the grain such as grain hardness, protein content, wet gluten, dough development time, dough stability, gliadin and glutenin contents between Yangmai-15 and Yangmai-16, and the results were significantly different. Notably, 216 and 197 protein spots were separated from Yangmai-15 and Yangmai-16, respectively. The isoelectric points of the identified proteins ranged from 4 to 10 and the molecular weights of proteins varied from 10 to 100 k Da. Further,21 and 8 specific differential protein spots were identified from the flour of Yangmai-15 and Yangmai-16, respectively.The surface properties of identified peptides consisted of hydrophobic or hydrophilic residues, as well as randomly scattered residues. The proteomic analysis of the wheat endosperms provides a novel insight into the biochemical basis for the differences in physicochemical properties between the soft and hard wheat varieties.

论文目录

• 1. Introduction
• 2. Materials and methods
•   2.1. Materials
•   2.2. Analysis of the physicochemical properties of the wheat flour
•   2.3. Protein extraction
•   2.4. Two-dimensional electrophoresis and image analysis
•   2.5. Trypsin digestion,characterization,and identification of proteins
•   2.6. Distribution of the hydrophobic and hydrophilic residues
•   2.7. Statistical analysis
• 3. Results and discussion
•   3.1. Characteristics of the flour
•   3.2. Two-dimensional electrophoresis analysis of wheat endosperm proteins
•   3.3. Analysis of the differential expression of proteins
•   3.4. Amino acid distribution of identified proteins
• 4. Conclusion
• Declaration of Competing Interest

文章来源

类型: 期刊论文

作者: Feng Jia,Haicheng Yin,Qi Wang,Yu Chen,Jinshui Wang

来源: Grain & Oil Science and Technology 2019年02期

年度: 2019

分类: 农业科技,工程科技Ⅰ辑

专业: 轻工业手工业

单位: College of Biological Engineering, Henan University of Technology

基金: the financial support of the National Natural Science Foundation of China(No.31771897,31871852,and 31772023)

分类号: TS201.21

页码: 27-32

总页数: 6

文件大小: 2097K

下载量: 7