Simultaneous Determination of Four Amino Acids in Fufang Ejiao Buxue Granule by HPLC

论文摘要

[Objectives] To establish an High Performance Liquid Chromatography（HPLC） method for simultaneous determination of four amino acids in Fufang Ejiao Buxue Granule. [Methods] The quantitative analysis was carried out with a Waters Sunfire C18 column（4.6 mm × 250 mm, 5 μm）. A binary mobile solvent was used: mobile solvent A was acetonitrile-0.1 mol/L sodium acetate solution（adjusting pH to 6.5 with 36% acetic acid）（7∶93） and mobile solvent B was acetonitrile-H2O（4∶1）. The mobile phase was delivered at a flow rate of 1.0 mL/min with a gradient elution profile（0-13 min, 100% A → 93% A; 13-17.9 min, 93% A → 88% A; 17.9-29.0 min, 88% A → 85% A; 29-39 min, 85% A → 66% A; 39-45 min, 66% A → 0% A）. The column temperature was at 43 ℃. The detection wavelength was 254 nm. [Results] The injection volume of L-hydroxyproline, glycine, alanine, and L-proline showed a good linear relationship with the chromatographic peak area in the range of 0.012 to 0.117, 0.022 to 0.218, 0.010 to 0.097, 0.016 to 0.160 μg, separately. The average recovery rate（n=6） was 96.4%, 97.3%, 97.1%, and 99.4%, respectively; the relative standard deviations were 1.2%, 1.9%, 1.7%, and 0.9%, respectively. [Conclusions] This method is simple in operation and good in reproducibility, and provides a reliable method for controlling the quality of Fufang Ejiao Buxue Granules.

论文目录

• 1 Introduction
• 2 Materials
• 3 Methods and results
•   3.1 Preparation of the solution
•     3.1.1 Preparation of mixed reference substance solution.
•     3.1.2 Preparation of test solution.
•     3.1.3 Preparation of negative control substance solution.
•     3.1.4 Preparation of derivatized solution.
•   3.2 Chromatographic conditions and system suitability test
•   3.3 Verification of the content determination methodology
•     3.3.1 Linear relationship test.
•     3.3.2 Precision test.
•     3.3.3 Stability test.
•     3.3.4 Reproducibility test.
•     3.3.5 Sample recovery rate test.
•   3.4 Determination of samples
• 4 Discussions
•   4.1 Test of chromatographic column
•   4.2 Test of sampling amount
•   4.3 Analysis of test results

文章来源

类型: 期刊论文

作者: Ningning YIN,Dongxiao GUO

来源: Medicinal Plant 2019年05期

年度: 2019

分类: 农业科技,工程科技Ⅰ辑,医药卫生科技

专业: 化学,中药学

单位: Shandong Institute for Food and Drug Control

分类号: R286.0;O657.72

DOI: 10.19600/j.cnki.issn2152-3924.2019.05.006

页码: 20-22+49

总页数: 4

文件大小: 284K

下载量: 12