论文摘要
Mitochondrial DNA（mtDNA）-encoded subunits of the oxidative phosphorylation（OXPHOS） complexes are synthesized in mitochondria. Initiation of translation of mtDNA-encoded subunits requires two general factors m IF2 and m IF3, whose counterparts in bacteria are essential for protein synthesis. In this study, we report the characterization of the fission yeast Schizosaccharomyces pombe m IF2（Mti2） and m IF3（Mti3）. Unlike its Saccharomyces cerevisiae counterpart（IFM1）, which is essential for respiratory growth, S. pombe cells lacking mti2 were able to grow on nonfermentable carbon sources, although they showed a slow growth phenotype as compared with wild-type（WT） cells. The growth defect of the mti2 deletion mutant could be suppressed by expressing IFM1, demonstrating functional conservation between the two proteins. Similarly, unlike its counterpart in S.cerevisiae（AIM23）, deletion of mti3 did not affect cell growth on respiratory media and mitochondrial translation. However, deletion of mti3 exacerbated the growth defect of the（35） mti2 mutant,suggesting that the two proteins had distinct, but partially overlapping functions during mitochondrial translational initiation in S. pombe. Sucrose gradient sedimentation analyses showed that both Mti2 and Mti3 associated with the small subunit of the mitochondrial ribosome. Our findings support the view that the importance of the mitochondrial translation initiation factors varies among the organisms.
论文目录
文章来源
类型: 国内会议
作者: 罗颖,汪一荣,苏茹月,谢婉秋,刘泽铖,黄鹰
关键词: 酵母,线粒体,蛋白翻译
来源: 多彩菌物 美丽中国——中国菌物学会2019年学术年会 2019-08-03
年度: 2019
分类: 基础科学
专业: 生物学,生物学
单位: 南京师范大学
分类号: Q933
DOI: 10.26914/c.cnkihy.2019.002145
页码: 232
总页数: 1
文件大小: 786k
下载量: 5
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