The Light Regime Effect on Triacylglycerol Accumulation of Isochrysis zhangjiangensis

论文摘要

Stress state of microalgal cells is caused under unfavorable conditions such as disordered light regime and depleted nitrogen. The stress state can impair photosynthetic efficiency, inhibit cell growth and result in the accumulation of triacylglycerol（TAG） from protective mechanisms. Continuous light or nitrogen starvation was applied on microalgae and performed effectively on inducing TAG production. To evaluate the light regime effect on inducing TAG production, the effect of different light regimes on nitrogen-starved Isochrysis zhangjiangensis was investigated in this work. The continuous light and nitrogen starvation elevated TAG content of biomass by 73% and 193%, respectively. Furthermore, the TAG accumulation of I. zhangjiangensis cell under nitrogen starvation decreased under aggravated stress from continuous illumination. Our results demonstrated that culturing the cells with 14 L: 10 D light regime under nitrogen starvation is the optimal mode to achieve maximal accumulation of TAG. A recovery in light regime was necessary for I. zhangjiangensis cultivation.

论文目录

• 1 Introduction
• 2 Materials and Methods
•   2.1 Strains and Culture Conditions
•   2.2 Light Regimes and N-Deprivation Effect
•   2.3 Data Measuring Method
• 3 Results and Discussion
•   3.1 Effect of Continuous Light Acclimation on I.zhangjiangensis seeds
•   3.2 Effect of Light Regime on Cell Growthof I.zhangjiangensis
•   3.3 Effect of Light Regime on Photosynthetic Activ-ity of I.zhangjiangensis
•   3.4 Effect of Light Regime on TAG Accumulation ofI.zhangjiangensis
•   3.5 Recovery of I.zhangjiangensis Cells fromPhotodamage During the Dark Time
• 4 Conclusions

文章来源

类型: 期刊论文

作者: FAN Xuran,CAO Xupeng,CHU Yadong,WU Peichun,XUE Song

来源: Journal of Ocean University of China 2019年02期

年度: 2019

分类: 基础科学

专业: 生物学

单位: Marine Bioengineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences,University of Chinese Academy of Sciences

基金: supported by the National High Technology Research and Development Program ‘863’ (No. 2012 AA052101),the Hundred Talent Program of the Chinese Academy of Sciences (No. A1097)

分类号: Q945

页码: 474-480

总页数: 7

文件大小: 606K

下载量: 9