论文摘要
In filamentous fungi, 1,8-dihydroxynaphthalene（DHN） melanin is a major component of the extracellular matrix, endowing fungi with environmental tolerance, and some pathogenic species with pathogenicity. However, the cytological mechanism of the melanin biosynthesis pathway remains obscure. Using the gray mold pathogen Botrytis cinerea, six key enzymes participate in DHN melanin synthesis, including polyketide synthases Bc PKS12/13, hydrolase Bc YGH1, 1,3,6,8-tetrahydroxynaphthalene（THN） reductases BcBRN1/2, and scytalone dehydratase Bc SCD1. We identified that the ?bcscd1 mutant accumulated scytalone in culture filtrate rather than in mycelium. Excessive scytalone appears to be self-toxic to the fungus, leading to repressed sclerotial germination and sporulation in the ?bcscd1 mutant. The BcBRN1/2 enzymes responsible for synthesizing scytalone were localized in endosomes and further trafficked to the cell surface. The BcSCD1 accumulated on the cell wall, being consistent with extracellular occurrence of its precursor scytalone. Moreover, the early stage melanin synthesis enzymes, including the BcPKS12/13 and BcYGH1, were localized in peroxisome vesicles. This study revealed the sub-cellular mechanisms of melanin biosynthetic enzymes in B. cinerea, indicating that encapsulation and externalization of the melanin synthetic enzymes should be harmoniously organized, most likely to assure enzymatic efficiency, and to avoid the adverse effect of the intermediate.
论文目录
文章来源
类型: 国内会议
作者: Xue Chen,Yantao Na,Pinkuan Zhu,Yiwen Wang,Yina Jiang,Yifan He,Chenghua Zhang,Dandan Ren,Han Guo,Sheng Xiang,Qin Li,Ling Xu
来源: 第三届全国植物开花·衰老与采后生物学大会 2019-11-04
年度: 2019
分类: 基础科学,农业科技
专业: 生物学,农业基础科学,植物保护
单位: School of Life Sciences, East China Normal University
分类号: S432.44
DOI: 10.26914/c.cnkihy.2019.003355
页码: 69
总页数: 1
文件大小: 558k
下载量: 15
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