Profiling of microRNA-like RNAs associated with sclerotial development in Sclerotinia sclerotiorum

论文摘要

Sclerotinia sclerotiorum is one of the most common plant fungal pathogens,which causes great economic losses worldwide.Sclerotium is a dormant structure that plays significantly biological and ecological roles in the life cycle of S.sclerotiorum and other species of sclerotium-forming fungi.Sclerotial development is regulated by complicated pathways involving gene regulation on post-transcriptional level.MicroRNA-like small RNA（milRNA） is a non-coding small RNA that plays a regulatory role in the post-transcriptional level in fungi.In this study,candidate regulatory milRNAs were investigated in S.sclerotiorum at the differential stages of sclerotial development.The profiles of small RNA from sclerotia sampled from three representative developmental stages were investigated by high-throughput sequencing.A total of 275 milRNAs were identified,of which 243 were predicted as novel milRNAs.Among them,47 milRNAs were differential expression.The target RNAs of these 47 milRNAs were in silico analyzed for their putative functions that were mainly on cellular process,metabolic process,organelle part and catalytic activity.In order to validate the sequencing results,the expression levels of 9 milRNAs and their targets were analyzed by qRT-PCR assays.The results showed that Ss-milR-3 was found to be upregulated as well as the corresponding target RNA showed down-regulated at the development and maturation stages of sclerotial formation.This study will facilitate the better understanding of the milRNA regulation associated with sclerotial development in S.sclerotiorum and other sclerotium-forming fungi.

论文目录

文章来源

类型: 国内会议

作者: 王泽昊,Xia Zihao,Tian Jiamei,Ding Chengsong,梁月

来源: 中国植物病理学会2019年学术年会 2019-07-20

年度: 2019

分类: 基础科学,农业科技

专业: 生物学,农业基础科学,植物保护

单位: Collage of Plant Protection,Shenyang Agricultural University

分类号: S432.44

DOI: 10.26914/c.cnkihy.2019.020109

页码: 87

总页数: 1

文件大小: 50k

下载量: 5

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