论文摘要
Ralstonia solanacearum,a soilborne bacterial pathogen,is the causal agent of bacterial wilt and infects over 200 plant species in 50 families.The pathogen is lethal to many solanaceous species,including eggplant,tobacco and peanut.The pathogen is notorious for its genetic variations,and the regulatory mechanism that governs the pathogenicity of R.solanacearum is not well—understood.In this study,we set to develop a convenient and reliable delivery method for stable and directed deletion of gene or DNA sequence in the genome of R.solanacearum.The method is modified from the suicide vector pRC,which was developed previously for gene knock—in at a permissive site downstream of glmS in the genome of R.solanacearum strain GMI1000 through homologous recombination.The modified gene deletion system consists of a fusion DNA fragment containing the upstream and downstream region of a target gene and a gentamycin resistance gene as a selection marker,as well as a growth medium which primes R.solanacearum to a state prone to receiving DNA fragments for integration.The feasibility of this gene deletion system was tested by targeting a regulatory gene in R.solanacearum EP1,which is a virulent strain isolated from the diseased eggplant in Guangdong Province of China.The results showed that the resultant mutants displayed much reduced EPS production.Development of such a convenient gene deletion system would facilitate further investigations of the pathogenic mechanisms of this important bacterial pathogen.
论文目录
文章来源
类型: 国内会议
作者: Yan Jinli,Zhang Lianhui
来源: 中国植物病理学会2019年学术年会 2019-07-20
年度: 2019
分类: 基础科学,农业科技
专业: 生物学,植物保护
单位: Guangdong Province Key Laboratory of MicrobialSignals and Disease control,Integrative Microbiology Research Centre,South China Agricultural University
分类号: S432.1
DOI: 10.26914/c.cnkihy.2019.020369
页码: 354
总页数: 1
文件大小: 44k
下载量: 8
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