论文摘要
Sclerotinia sclerotiorum（Lib.） de Bary is a broad host range plant pathogen and causes lots of loss in the worldwide scale every year.Sclerotinia sclerotiorum can form the infection structure,named comprounded appressorium,in order to adhere and penetrate the host plant during pathogenic processes.In addition,Sclerotinia sclerotiorum is able to synthesize oxalic acid and cell wall degrading enzymes,which play important role in resisting host defense reaction and accelerating pathogenicity.CFEM（common in several fungal extracellular membrane proteins） domain is unique in fungi and contains eight conserved cysteine residues.InMagnaporthe oryzae,the CFEM domain of seven-transmembrane protein Pth11,a significant G-protein-coupled receptor,is necessary for proper development of appressoria,appressorialike structures and pathogenicity.However,the underlying mechanisms by which CFEM domain-containing proteins act remain largely unknown,especially in broad-hostrange necrotrophic pathogen 5.Sclerotiorum.The transcript level of SsCFEM1 increased highly at 24 hours post inoculation（hpi） and then maintained at a high level expression during late stages（24～48 hpi）,indicating that SsCFEM1 may be involved in infection of S.sclerotiorum.In order to futher investigate the role of SsCFEM1 in S.Sclerotiorum,the knock-out mutant was obtained by PEG-mediated protoplast transformation.The knock-out mutant showed no significant changes on mycelial growth,colony morphology and sclerotial development compared with wild type strain.Although the knock-out mutant can form normal comprounded appressorium,the number of it is significantly reduced.Virulence of knock-out mutants was significant declined on detached leaves on Nicotiana benthamiana,suggesting that SsCFEM1 is involved in the virulence of S.sclerotiorum.Therefore,our data suggested that SsCFEM1 contributes to virulence and comprounded appressorium production.
论文目录
文章来源
类型: 国内会议
作者: Ji Xu,Liu Jinliang,Zhang Yanhua,Zhang Xianghui,Pan Hongyu
来源: 中国植物病理学会2019年学术年会 2019-07-20
年度: 2019
分类: 基础科学,农业科技
专业: 生物学,植物保护
单位: College of Plant Sciences,Jilin University
分类号: S432.1
DOI: 10.26914/c.cnkihy.2019.020093
页码: 60
总页数: 1
文件大小: 51k
下载量: 7
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