Extraction of electrons by magnetite and ferrihydrite from hydrogen-producing Clostridium bifermentans by strengthening the acetate production pathway

论文摘要

Conductive mineral nanoparticles, such as magnetite, can promote interspecies electron transfer between syntrophic partners.However, the effect of magnetite has only been inferred in intraspecific electron output. Herein, a hydrogen-producing strain,namely, Clostridium bifermentans, which holds several electron output pathways, was used to study the effect of magnetite on the intraspecific electron output manner. Additionally, insulated amorphous ferrihydrite, which was used as an extracellular electron acceptor, was selected to compare with magnetite. Electrons, which were originally used to generate hydrogen, were shunted with the addition of magnetite and ferrihydrite, which resulted in the reduction of hydrogen production and accumulation of Fe（II）. Interestingly, more electrons（39.7% and 53.5%） were extracted by magnetite and ferrihydrite, respectively, which led to less production of butyrate and more acetate. More importantly, the increased electron extraction efficiency suggested that electroactive microorganisms can switch metabolic pathways to adapt to electron budget pressure in intraspecific systems. This work broadens the understanding of the interaction between iron oxides and fermentative hydrogen-producing microbes that hold the capacity of Fe（III） reduction.

论文目录

• 1 Introduction
• 2 Materials and methods
•   2.1 Microorganism and culture medium
•   2.2 Preparation of magnetite and ferrihydrite
•   2.3 Experimental procedures
•   2.4 Chemical analysis
•   2.5 Electrochemical characterization
•   2.6 Statistical analysis
• 3 Results and discussion
•   3.1 The adverse effect of magnetite and ferrihydrite on hydrogen production
•   3.2 The stimulation of electron output efficiency by magnetite and ferrihydrite
•   3.3 The enhancement of the acetate production path-way
•   3.4 Electrochemical activity influenced by magnetite and ferrihydrite
• 4 Conclusions

文章来源

类型: 期刊论文

作者: ZHANG YueChao,LIU FangHua,XU HengDuo,XIAO LeiLei

来源: Science China(Technological Sciences) 2019年10期

年度: 2019

分类: 工程科技Ⅱ辑,工程科技Ⅰ辑

专业: 无机化工

单位: Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences,Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology,University of Chinese Academy of Sciences

基金: supported by the National Natural Science Foundation of China(Grant Nos.91751112,41573071,41703075,41807325),the Senior User Project of RV KEXUE(Grant No.KEXUE2018G01),the Key Research Project of Frontier Science(Grant No.QYZDJ-SSW-DQC015)of the Chinese Academy of Sciences,the Natural Science Foundation(Grant Nos.JQ201608,ZR2016DQ12),the Young Taishan Scholars Program(Grant No.tsqn20161054)of Shandong Province

分类号: TQ116.2

页码: 1719-1725

总页数: 7

文件大小: 453K

下载量: 10