单核细胞增多性李斯特菌RsbR抗酸应激机制（英文）

论文摘要

目的:探明单增李斯特菌RsbR的两个保守磷酸化位点是否影响SigB（sigma B）的表达。创新点:解析了RsbR在单增李斯特菌中SigB网络调控中的作用。方法:通过基因缺失、定点突变和回补技术构建rsb R基因缺失株、回补株和回补突变菌株,进行酸应激条件下的生长能力和强酸条件下的存活能力试验,并检测在酸应激下菌株中的SigB基因和蛋白表达的改变情况。结论:Rsb R缺失能够显著降低单增李斯特菌的抗酸应激能力和下调SigB表达,Rsb R-175位点是SigB调控途径中Rsb T释放的先决条件。

论文目录

• 1 Introduction
• 2 Materials and methods
•   2.1 Bacterial strains, plasmids, and culture conditions
•   2.2 Bioinformatics analysis
•   2.3 Construction of the rsbR deletion mutant
•   2.4 Complementation of the rsbR deletion mutant
•   2.5 Site-directed mutagenesis
•   2.6 Growth and survival assays
•   2.7 Acidic stress for analysis of SigB expression
•   2.8 qRT-PCR and western blotting
•   2.9 Statistical analysis
• 3 Results
•   3.1 Identification of the rsbR deletion mutant and complemented strains
•   3.2 Effects of L.monocytogenes rsbR on growth and survival under acidic conditions
•   3.3 Role of residue T175 contributing to RsbR function in listerial survival under acidic stress
•   3.4 Regulaton of RsbR and its residue T175 of SigB expression under stress conditions
• 4 Discussion
• Contributors
• Compliance with ethics guidelines

文章来源

类型: 期刊论文

作者: Ke HE,Yong-ping XIN,Ying SHAN,Xian ZHANG,Hou-hui SONG,Wei-huan FANG

关键词: 单增李斯特菌,抗酸应激

来源: Journal of Zhejiang University-Science B（Biomedicine & Biotechnology） 2019年08期

年度: 2019

分类: 工程科技Ⅱ辑,基础科学,农业科技

专业: 生物学,畜牧与动物医学

单位: College of Animal Science and Technology, Zhejiang A&F University,Zhejiang University Institute of Preventive Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine

基金: Project supported by the National Natural Science Foundation of China(Nos.31702250 and 31600292),the Open Fund Project by Key Laboratory of Animal Preventive Medicine of Zhejiang Province(No.ZPKLPVM2017KFKT001),China

分类号: S852.61

页码: 660-669

总页数: 10

文件大小: 2041K

下载量: 41