虾壳中虾青素及虾青素酯的高效液相测定（英文）

论文摘要

建立一种虾壳中虾青素及虾青素酯的高效液相测定法。鲜虾壳用5%盐酸除钙离子,烘干后用无水乙醇提取。将虾壳的无水乙醇提取液与硫酸铵水溶液混合进行双水相萃取虾青素,收集分布在双水相中间层的虾青素粗品。向收集的虾青素粗品中加入蒸馏水离心,重复几次,收集沉淀并吹干。将干燥的虾青素粗品用丙酮溶解,进行薄层色谱分离,分离出的色素带取出用丙酮溶解,再进行HPLC测定。结果表明,3 mL虾青素乙醇提取液/4.5 mL 20%硫酸铵溶液双水相体系可用来制备虾青素粗品;虾青素在乙醇溶液中最大吸收峰波长为472 nm。虾青素粗品通过薄层色谱可分离出游离虾青素、虾青素单酯、虾青素双酯、海胆酮等色素,根据高效液相色谱图可知,游离虾青素出峰时间为4 min～5.5 min,虾青素单酯得出峰时间为10.5 min～27.8 min;该方法对样品的前处理简便,适合对虾壳中虾青素及虾青素酯含量的测定。

论文目录

• 1 Introduction
•   1.1 Astaxanthin and astaxanthin esters
•   1.2 The existence form and the bioactivity of astaxanthin
•   1.3 Research status of astaxanthin and astaxanthin esters
•   1.4 The content and purposes of research
• 2 The experimental steps
•   2.1 Pretreatment of shrimp shell
•   2.2 The preparation of astaxanthin
•   2.3 Determination of astaxanthin by thin layer chromatography
•   2.4 Determination of astaxanthin by high performance liquid chromatography
•     2.4.1 Drawing a standard curve
•     2.4.2 Determination of Astaxanthin and Astaxanthin esters by HPLC
•   2.5 Calculation of astaxanthin and astaxanthin esters content
• 3 Experimental results and analysis
•   3.1 Astaxanthin extraction
•   3.2 Thin layer chromatography
•   3.3 Determination of the astaxanthin standard and the standard curve by HPLC
•     3.3.1 HPLC of astaxanthin standard
•     3.3.2 Astaxanthin standard curve
•   3.4 HPLC determination of astaxanthin and astaxanthin esters
•     3.4.1 Crude astaxanthin
•     3.4.2 HPLC diagrams of pigment bands
• 4 Discussion

文章来源

类型: 期刊论文

作者: 李帅,郭俊如,冯金华,朱泽民,李玲莉,郭晓芳,隋汶燕,王萌,左文婷

关键词: 虾青素,虾青素酯,双水相,薄层色谱分离,高效液相色谱

来源: Marine Science Bulletin 2019年02期

年度: 2019

分类: 基础科学,工程科技Ⅰ辑

专业: 化学,轻工业手工业

单位: 大连海洋大学海洋科技与环境学院,河北农业大学海洋学院

基金: supported by Liao Ning Revitalization Talents Program No. XLYC1807161,Dalian High-level Talents Innovation Support Plan No. 2017RQ063,Dalian Ocean University Zhanlan scholar Program,The National Natural Science Foundation of China under contract Nos. 41206013,41430963,the Public Science and Technology Research Funds Projects of Ocean under contract No. 201205018,the National Science and Technology Support Program under contract No. 2014BAB12B02,Projects of Institute of Marine Industry Technology of Liaoning Universities

分类号: TS254.9;O657.72

页码: 57-71

总页数: 15

文件大小: 1649K

下载量: 140