论文摘要
Mosquito saliva has been reported to include many immune active molecules, such as C-type lectin, peptidoglycan recognition protein and lysozyme, which participate in the maintenance of mosquito’s immune defense. 34k2 is a secreted protein which was found highly and specifically expressed in the saliva of Aedes albopictus mosquitoes. This study focused on the possible effects of recombinant salivary specific 34k2 protein（rAlb-3k42） of A. albopictus on recognizing Escherichia coli, Staphylococcus aureus and other bacteria, and its effects on the growth of E. coli. The results showed that r Alb-34k2 protein could bind to E. coli, A. baumannii, S. aureus and P. aeruginosa, but not to β-hemolytic streptococcus via bacterial binding assay. Moreover, the growth curves of E. coli which was treated with high concentrations of rAlb-34k2 protein（137 ng/μL and 112 ng/μL） were found smoothly gentle and its OD600 ranged from 0.063 to 0.294 and 0.097 to 0.349 within 24 hours, respectively, which was unrelated to Ca2+. Conversely, the rAalb-CTL2 protein（a C-type lectin from recombinant saliva protein of A. albopictus） could promote the growth of E. coli in a manner of Ca2+ dependence. These data reveals that the rAlb-34k2 protein in the saliva of A. albopictus may act as a pattern recognition receptor of mosquitoes, which can recognize several kinds of bacteria and also inhibits the growth of E. coli in dose-dependent manner.
论文目录
文章来源
类型: 国际会议
作者: Tian Y.,Xu Q.,Jin R.,Zhu Y.,Shang Z.,Wang Z.,Cheng J.,Wu J.
来源: 第十七届国际免疫学大会 2019-10-19
年度: 2019
分类: 基础科学,医药卫生科技
专业: 生物学,基础医学
单位: Guizhou Medical University, Department of ImmunologyGuizhou Medical University, Department of Human ParasitologyGuizhou Medical University, Characteristic and Key Laboratory of Modern Pathogen BiologyGuizhou Medical University, Microbiology and Biochemical Pharmacy Engineering Center
分类号: R384.1
DOI: 10.26914/c.cnkihy.2019.005713
页码: 72
总页数: 1
文件大小: 293k
下载量: 2