Understanding gaseous reduction in swine manure resulting from nanoparticle treatments under anaerobic storage conditions

论文摘要

Manure is an impending source of carbon（C）, sulfur（S） and water（H2 O）. Consequently,microbial populations utilize these constituents to produce methane（CH4）, carbon dioxide（CO2）, greenhouse gases（GHGs）, and hydrogen sulfide（H2 S）. Application of nanoparticles（NPs） to stored manure is an emerging GHG mitigation technique. In this study, two NPs: nano zinc oxide（nZnO） and nano silver（nAg） were tested in swine manure stored under anaerobic conditions to determine their effectiveness in mitigating gaseous emissions and total gas production. The biological sources of gas production, i.e., microbial populations were characterized via Quantitative Polymerase Chain Reaction（qPCR） analysis. Additionally, pH, redox, and VFAs were determined using standard methods. Each treatment of the experiment was replicated three times and NPs were applied at a dose of 3 g/L of manure. Also, headspace gas from all treatment replicates were analyzed for CH4 and CO2 gas concentrations using an SRI-8610 Gas Chromatograph and H2 S concentrations were measured using a Jerome 631 X meter. Nanoparticles tested in this study reduced the cumulative gas volume by 16%–79% compared to the control. Among the NPs tested, only nZnO consistently reduced GHG concentrations by 37%–97%. Reductions in H2 S concentrations ranged from 87% to97%. Gaseous reductions were likely due to decreases in the activity and numbers of specific gas producing methanogenic archaea and sulfate reducing bacterial（SRB）species.

论文目录

• Introduction
• 1. Materials and methods
•   1.1. Manure collection and characterization
•   1.2. Nanoparticles used and their application methods
•     1.2.1. Direct application method of nanoparticles
•     1.2.2. Preparation of sodium alginate beads for indirect applica-tion of nanoparticles
•   1.3. Experimental set-up
•   1.4. Total coliform bacterial population determination
•   1.5. Anaerobic bacterial population determination
•   1.6. DNA isolation and preparation for q-PCR
•   1.7. Manure mineral analysis
•   1.8. Statistical analysis
• 2. Results and discussion
•   2.1. Effects of nanoparticles on manure pH and redox potential
•   2.2. Effects of nanoparticles on manure volatile fatty acids
•   2.3. Effects of nanoparticles on cumulative gas production
•   2.4. Effects of nanoparticles on carbon dioxide concentration
•   2.5. Effects of nanoparticles on methane concentration
•   2.6. Effects of nanoparticles on hydrogen sulfide concentration
•   2.7. Effects of nanoparticles on total coliform bacteria （aerobic）
•   2.8. Effects of nanoparticles on anaerobic bacteria
•   2.9. Effects of nanoparticles on methanogenic archaea
•   2.1 0. Effects of nanoparticles on H2S producing bacteria
•   2.1 1. Effect of nanoparticles on manure mineral contents
• 3. Implication of the present study
• 4. Conclusions

文章来源

类型: 期刊论文

作者: Niloy Chandra Sarker,Md Borhan,Ann-Marie Fortuna,Shafiqur Rahman

来源: Journal of Environmental Sciences 2019年08期

年度: 2019

分类: 工程科技Ⅰ辑,农业科技

专业: 材料科学,环境科学与资源利用,农业基础科学

单位: Agricultural and Biosystems Engineering Department, North Dakota State University,USDA-ARS, Grazinglands Research Laboratory

基金: The USDA National Institute of Food and Agriculture award#2015-67022-22996 supported this work,supported by the USDA National Institute of Food and Agriculture,Hatch project number ND01477

分类号: X713;TB383.1

页码: 179-191

总页数: 13

文件大小: 444K

下载量: 15