Separation and Purification of Monascus Pigments from Red Yeast Rice

Separation and Purification of Monascus Pigments from Red Yeast Rice

论文摘要

It is difficult to extract beneficial components from Monascus pigments due to their complex composition. The low purity of Monascus pigment preparations limits their further application in research and production. Therefore, this study was conducted to optimize the conditions for extraction and isolation of Monascus pigments from red yeast rice, to improve the purity of Monascus pigment preparations. Three fractions were isolated using column chromatography from red yeast rice, they were red, orange and yellow respectively. Then, the three fractions were analyzed for composition and purity using thin layer chromatography and liquid chromatography-mass spectrometry(LC-MS). The chromatograms revealed that the yellow and orange fractions had complex compositions that were difficult to be separated, while the red fraction consisted of fewer components. Finally, a single component was isolated from the red fraction using a thin layer chromatography plate, identified as monascorubramine according to its molecular mass.

论文目录

  • Materials and Methods
  •   Materials and reagents
  •   Instruments and equipment
  •   Extraction of Monascus pigment
  •   Separation of Monascus pigments by column chromatography
  •     Pretreatment of silica gel
  •     Sample processing
  •     Column preparation
  •     Sample loading
  •     Elution
  •   Detection and separation of Monascus pigments by thin layer chromatography
  •   Determination of Monascus pigments by high performance liquid chromatography
  •     Pretreatment of sample
  •     Chromatographic conditions
  •   Determination of Monascus pigments by mass spectrometry
  • Results and Analysis
  • Conclusions
  • 文章来源

    类型: 期刊论文

    作者: Tongle LI,Xiuhe LIU,Li YAN

    来源: Agricultural Biotechnology 2019年02期

    年度: 2019

    分类: 农业科技,工程科技Ⅰ辑

    专业: 轻工业手工业

    单位: Qilu University of Technology(Shandong Academy of Sciences)

    分类号: TS213.3

    DOI: 10.19759/j.cnki.2164-4993.20190410.001

    页码: 177-178+184

    总页数: 3

    文件大小: 412K

    下载量: 59

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